Metabolism of deoxyribonucleotides. I. Purification and properties of deoxycytidine monophosphokinase of calf thymus.

Deoxycytidine kinase. II. Purification and general properties of the calf thymus enzyme.

Deoxycytidine kinase has been purified 500-fold from calf thymus. It is concluded that the enzyme will catalyze the phosphorylation of deoxycytidine, 1-P-~arabinofuranosylcytosine, deoxyguanosine, and deoxyadenosine since the protein catalyzing these activities showed coincident purification. Also, this protein could not be further fractionated by isoelectric focusing, gel filtration, or sucros...

Enzymatic Synthesis of Deoxyribonucleotides * V. PURIFICATION AND PROPERTIES OF THIOREDOXIN REDUCTASE FROYI ESCHERICHIA COLI B E. COLLEEN MOORE,~ PETER REICHARD,~ AND LARS THELANDER

The preceding paper (1) described the purification from Escherichia coli of thioredoxin, a heat-stable, small protein involved in the reduction of cytidine diphosphate to deoxycytidine diphosphate by the cytidine diphosphate reductase system isolated from E. coli (2). Evidence was presented that thioredoxin was first reduced enzymatically with TPNH to a sulfhydryl form (thioredoxin-(SH)J, which...

Glutathione-dependent hydrogen donor system for calf thymus ribonucleoside-diphosphate reductase.

Purified calf thymus ribonucleoside-diphosphate reductase (2'-deoxyribonucleoside-diphosphate:oxidized-thioredoxin 2'-oxidoreductase, EC 1.17.4.1), showed an absolute requirement for a dithiol as hydrogen donor, whereas the natural monothiol glutathione (GSH) was inactive per se. However, a protein partially purified from thymus coupled the oxidation of GSH to the formation of deoxyribonucleoti...

Purification and properties of calf thymus casein kinases I and II.

Mechanism of Calf thymus DNA radioprotection by sucrose: A combined effect of scavenging action and altered water

Background: Development of safe radioprotector is a challenging task. In this study radioprotective effect of sucrose has been demonstrated in calf thymus DNA (CtDNA). Sucrose is a free radical scavenger and also acts as osmolyte and therefore can influence the water activity around DNA and effects of radiation on DNA. Hoechst 33258 was used to probe the possible alteration in physicochemical p...